The ongoing research in the Sharma Lab at Ball State University delves into the intricate cellular and molecular processes governing coronary angiogenesis during both cardiac development and regeneration. Central to our investigations is the pivotal role of Aplnr, a G-protein coupled receptor, alongside the influence of hypoxia and the transcription factor Sox17 in orchestrating the regulation of coronary angiogenesis. Our research endeavors encompass a comprehensive array of methodologies spanning both in vivo and in vitro domains, allowing for a holistic understanding of this complex phenomenon. Leveraging a mouse model system in conjunction with endothelial and cardiomyocyte cell cultures, as well as 3D whole organ and explant cultures, we aim to elucidate the intricate interplay between these molecular players in driving coronary angiogenesis. Employing cutting-edge molecular biology techniques including quantitative real-time polymerase chain reaction (qRT-PCR), western blotting, and RNA sequencing, we delve deep into the genetic and molecular underpinnings of angiogenic processes. Furthermore, histological and microscopy approaches are employed to visualize and characterize the structural and cellular dynamics involved in coronary angiogenesis. By integrating these multidisciplinary approaches, our research seeks to unravel the fundamental mechanisms underpinning coronary angiogenesis, offering insights that may ultimately inform novel therapeutic strategies for cardiac regeneration and disease intervention.